Plant Cell Culture Strategies for the Production of Terpenes as Green Solvents


Plant Cell Culture Strategies for the Production of Terpenes as Green Solvents

Mubarak Ali Khan, Tariq Khan, Huma Ali

Green chemistry implies the synthesis and design of fine chemicals of biological origin which can be used in industrial applications. Petroleum-based solvents such as n-hexane and toluene, etc. used in different extraction procedures have shown adverse effects on human health and the environment. The alternatives are the terpenes derived from essential oils have represented their potential as green solvents. The practical use of d-limonene and α-pinene in the extraction of oil from various substances has recently, been investigated as an alternative to petro based solvents. These volatiles are mainly produced in different parts of medicinal and aromatic plants. However, their limited productivity due to geographic variability and environmental fluctuations in natural plants does not meet the emerging industrial demands. In this chapter, the potential of different plant cell culture approaches for the enhancement of production of important terpene volatiles in different plants have been elucidated. Biotechnological methods which can improve the yield of essential oils through genetic engineering of the metabolic pathways responsible for the biosynthesis of terpene volatiles have been described with recent examples.

Essential Oils, Volatiles, Green Solvents, Terpenes, Cell Cultures, Callus Cultures

Published online 5/25/2019, 20 pages

Citation: Mubarak Ali Khan, Tariq Khan, Huma Ali, Plant Cell Culture Strategies for the Production of Terpenes as Green Solvents, Materials Research Foundations, Vol. 50, pp 1-20, 2019


Part of the book on Industrial Applications of Green Solvents

[1] Y. Li, A.S. Fabiano-Tixier, F. Chemat, Essential oils as green solvents, in: Y. Li, A.S. Fabiano-Tixier, F. Chemat, Essential oils as reagents in green chemistry, Springer, 2014, pp. 55-61.
[2] S. Bertouche, V. Tomao, A. Hellal, C. Boutekedjiret, F. Chemat, First approach on edible oil determination in oilseeds products using alpha-pinene, J. Essent. Oil Res. 25 (2013) 439-443.
[3] M. Virot, V. Tomao, C. Ginies, F. Visinoni, F. Chemat, Green procedure with a green solvent for fats and oils’ determination: microwave-integrated Soxhlet using limonene followed by microwave Clevenger distillation, J. Chrom. 1196 (2008) 147-152.
[4] E.M. Napoli, G. Curcuruto, G. Ruberto, Screening of the essential oil composition of wild Sicilian rosemary, Biochem. Systemat. Ecol. 38 (2010) 659-670.
[5] S. Chemat, V. Tomao, F. Chemat, Limonene as green solvent for extraction of natural products, Green Solvents I, Springer, 2012, pp. 175-186.
[6] T. Mulder-Krieger, R. Verpoorte, A.B. Svendsen, J. Scheffer, Production of essential oils and flavours in plant cell and tissue cultures. A review, Plant Cell Tissue Organ Cult. 13 (1988) 85-154.
[7] T. Khan, B.H. Abbasi, I. Iqrar, M.A. Khan, Z.K. Shinwari, Molecular identification and control of endophytic contamination during in vitro plantlet development of Fagonia indica, Acta Physiologiae Plantarum 40 (2018) 150.
[8] R. Rani, M.A. Khan, W.K. Kayani, S. Ullah, I. Naeem, B. Mirza, Metabolic signatures altered by in vitro temperature stress in Ajuga bracteosa Wall. ex. Benth, Acta physiologiae plantarum 39 (2017) 10.
[9] S. Saeed, H. Ali, T. Khan, W. Kayani, M.A. Khan, Impacts of methyl jasmonate and phenyl acetic acid on biomass accumulation and antioxidant potential in adventitious roots of Ajuga bracteosa Wall ex Benth., a high valued endangered medicinal plant, Physiology and Molecular Biology of Plants 23 (2017) 229-237.
[10] M. Zuzarte, L. Salgueiro, Essential oils chemistry, Bioactive essential oils and cancer, Springer, 2015, pp. 19-61.
[11] L.S. Nerio, J. Olivero-Verbel, E. Stashenko, Repellent activity of essential oils: a review, Bioresour. Technol. 101 (2010) 372-378.
[12] M.A. Khan, T. Khan, A. Nadhman, Applications of plant terpenoids in the synthesis of colloidal silver nanoparticles, Adv. Colloid Interface Sci. 234 (2016) 132-141.
[13] N. Sangwan, A. Farooqi, F. Shabih, R. Sangwan, Regulation of essential oil production in plants, Plant Growth Regul. 34 (2001) 3-21.
[14] S. Zwenger, C. Basu, Plant terpenoids: applications and future potentials, Biotechnol. Mol. Biol. Rev. 3 (2008) 1-7.
[15] H. Ali, M.A. Khan, N. Ullah, R.S. Khan, Impacts of hormonal elicitors and photoperiod regimes on elicitation of bioactive secondary volatiles in cell cultures of Ajuga bracteosa, J. Photochem. Photobiol. B Biol. 183 (2018) 242-250.
[16] Y. Li, A.S. Fabiano-Tixier, F. Chemat, Essential oils: from conventional to green extraction,in: Y. Li, A.S. Fabiano-Tixier, F. Chemat, Essential oils as reagents in Green Chemistry, Springer, 2014, pp. 9-20.
[17] P. Masango, Cleaner production of essential oils by steam distillation, Journal of Cleaner Production 13 (2005) 833-839.
[18] B.H. Abbasi, H. Ali, B. Yücesan, S. Saeed, K. Rehman, M.A. Khan, Evaluation of biochemical markers during somatic embryogenesis in Silybum marianum L, 3 Biotech 6 (2016) 71.
[19] L. Daviet, M. Schalk, Biotechnology in plant essential oil production: progress and perspective in metabolic engineering of the terpene pathway, Flavour and Fragrance Journal 25 (2010) 123-127.
[20] J. Dı́az, A. Bernal, F. Pomar, F. Merino, Induction of shikimate dehydrogenase and peroxidase in pepper (Capsicum annuum L.) seedlings in response to copper stress and its relation to lignification, Plant Science, 161 (2001) 179-188.
[21] T. Khan, B.H. Abbasi, M.A. Khan, M. Azeem, Production of biomass and useful compounds through elicitation in adventitious root cultures of Fagonia indica, Ind. crop. Prod. 108 (2017) 451-457.
[22] H. Ali, M.A. Khan, W.K. Kayani, T. Khan, R.S. Khan, Thidiazuron regulated growth, secondary metabolism and essential oil profiles in shoot cultures of Ajuga bracteosa, Ind. Crop. Prod. 121 (2018) 418-427.
[23] C.C. Giri, M. Zaheer, Chemical elicitors versus secondary metabolite production in vitro using plant cell, tissue and organ cultures: recent trends and a sky eye view appraisal, Plant Cell Tissue Organ Cult. 126 (2016) 1-18.
[24] P. Olgunsoy, S. Ulusoy, U.Ç. Akçay, Metabolite production and antibacterial activities of callus cultures from rosa damascena mill. petals, Marmara Pharmaceutical Journal 21 (2017).
[25] J.K. Holopainen, Can forest trees compensate for stress-generated growth losses by induced production of volatile compounds?, Tree physiology 31 (2011) 1356-1377.
[26] S. Zielińska, E. Piątczak, D. Kalemba, A. Matkowski, Influence of plant growth regulators on volatiles produced by in vitro grown shoots of Agastache rugosa (Fischer & CA Meyer) O. Kuntze, Plant Cell Tissue Organ Cult. 107 (2011) 161.
[27] N. Pourebad, R. Motafakkerazad, M. Kosari-Nasab, N.F. Akhtar, A. Movafeghi, The influence of TDZ concentrations on in vitro growth and production of secondary metabolites by the shoot and callus culture of Lallemantia iberica, Plant Cell Tissue Organ Cult. 122 (2015) 331-339.
[28] L. Bassolino, E. Giacomelli, S. Giovanelli, L. Pistelli, A. Cassetti, G. Damonte, A. Bisio, B. Ruffoni, Tissue culture and aromatic profile in Salvia dolomitica Codd, Plant Cell Tissue Organ Cult. 121 (2015) 83-95.
[29] Y. Gounaris, Biotechnology for the production of essential oils, flavours and volatile isolates. A review, Flavour and Fragrance Journal 25 (2010) 367-386.
[30] J.A.T. da Silva, Floriculture, ornamental and plant biotechnology, Global Science Books 2006.
[31] S.C. Roberts, Production and engineering of terpenoids in plant cell culture, Nat. Chem. Biol. 3 (2007) 387.
[32] A. Marco-Medina, J.L. Casas, In vitro multiplication and essential oil composition of Thymus moroderi Pau ex Martinez, an endemic Spanish plant, Plant Cell Tissue Organ Cult. 120 (2015) 99-108.
[33] S. Bhat, P. Maheshwari, S. Kumar, A. Kumar, Mentha species: in vitro regeneration and genetic transformation, Mol. Biol. Today 3 (2002) 11-23.
[34] P. Avato, I.M. Fortunato, C. Ruta, R. D’Elia, Glandular hairs and essential oils in micropropagated plants of Salvia officinalis L, Plant Science, 169 (2005) 29-36.
[35] V.R. Affonso, H.R. Bizzo, C.L.S. Lage, A. Sato, Influence of growth regulators in biomass production and volatile profile of in vitro plantlets of Thymus vulgaris L, J. Agr. Food Chem. 57 (2009) 6392-6395.
[36] M.L. Fauconnier, M. Jaziri, M. Marlier, J. Roggemans, J.-P. Wathelet, G. Lognay, M. Severin, J. Homes, K. Shimomura, Essential oil production by Anthemis nobilis L. tissue culture, J. Plant Physiol. 141 (1993) 759-761.
[37] F. Sadeghian, J. Hadian, M. Hadavi, A. Mohamadi, M. Ghorbanpour, R. Ghafarzadegan, Effects of exogenous salicylic acid application on growth, metabolic activities and essential oil composition of Satureja khuzistanica Jamzad, Journal of Medicinal Plants, 3 (2013) 70-82.
[38] L.E.F. Monfort, S.K.V. Bertolucci, A.F. Lima, A.A. de Carvalho, A. Mohammed, A.F. Blank, J.E.B.P. Pinto, Effects of plant growth regulators, different culture media and strength MS on production of volatile fraction composition in shoot cultures of Ocimum basilicum, Ind. Crop. Prod. 116 (2018) 231-239.
[39] M. Sökmen, J. Serkedjieva, D. Daferera, M. Gulluce, M. Polissiou, B. Tepe, H.A. Akpulat, F. Sahin, A. Sokmen, In vitro antioxidant, antimicrobial, and antiviral activities of the essential oil and various extracts from herbal parts and callus cultures of Origanum acutidens, J. Agr. Food Chem. 52 (2004) 3309-3312.
[40] S.M. Razavi, H. Arshneshin, A. Ghasemian, In vitro callus induction and isolation of volatile compounds in callus culture of Lallemantia iberica (M. Bieb.) Fisch. & C.A. MEY, Journal of Plant Process and Function 5 (2017).
[41] K.P. Singh, M. Bala, S.P. NAMITA, S. Kumari, Mutation breeding in roses: A review, Journal of Ornamental Horticulture 19 (2016) 55-74.
[42] D. Jawdat, H. Al-Faoury, A. Odeh, R. Al-Rayan, B. Al-Safadi, Essential oil profiling in callus of some wild and cultivated Daucus genotypes, Ind. Crop Prod. 94 (2016) 848-855.
[43] T.H. Kim, J.H. Shin, H.H. Baek, H.J. Lee, Volatile flavour compounds in suspension culture of Agastache rugosa Kuntze (Korean mint), J. Sci. Food Agr. 81 (2001) 569-575.
[44] R. De Alwis, K. Fujita, T. Ashitani, K.i. Kuroda, Volatile and non-volatile monoterpenes produced by elicitor-stimulated Cupressus lusitanica cultured cells, J. Plant. Physiol. 166 (2009) 720-728.
[45] F. Inabuy, J.T. Fischedick, I. Lange, M. Hartmann, N. Srividya, A.N. Parrish, M. Xu, R.J. Peters, B.M. Lange, Biosynthesis of diterpenoids in Tripterygium adventitious root cultures, Plant physiol. 175 (2017) 92-103.