Adsorption of proteins onto non-soluble polysaccharides matrixes: a friendly strategy to isolate enzymes with potential application for downstream processes


Adsorption of proteins onto non-soluble polysaccharides matrixes: a friendly strategy to isolate enzymes with potential application for downstream processes

Nadia Woitovich Valetti, M. Emilia Brassesc, Guillermo Alfredo Picó

Polysaccharides with electrically charged groups, polyelectrolytes (PE), have the capacity to form hydrogels under different experimental conditions, which act as ionic exchanges with high affinity to adsorb proteins. Presented in this chapter is a description of the current state of the technic on the use of these matrixes. Alginate, carrageenan, chitosan, pectin, etc. are the most used PE to make beds with the capacity of proteins adsorption. The presence of electrically charged residual groups in these PE allows their use as beds for the ion exchange chromatography in stirred tank of packed bed or expended bed. We demonstrated the adsorption of lysozyme, a model protein positively charged, onto Alginate-Guar gum matrixes cross-linked with epichlorohydrin (negatively charged). Their physical characterization, equilibrium isotherms and adsorption kinetics were carried out. Successive cycles of adsorption-washing-elution were performed. The results demonstrate the reversibility of the process and the capacity of this enzyme purification method.

Chromatography, Adsorption, Alg-guar gum, Polyelectrolytes, Bioseparation

Published online 4/25/2017, 23 pages
Copyright © 2016 by the author(s)
Published under license by Materials Research Forum LLC., Millersville PA, USA

Citation: Nadia Woitovich Valetti, M. Emilia Brassesc, Guillermo Alfredo Picó, ‘Adsorption of proteins onto non-soluble polysaccharides matrixes: a friendly strategy to isolate enzymes with potential application for downstream processes’, Materials Research Foundations, Vol. 15, pp 84-106, 2017


The article was published as article 4 of the book Applications of Adsorption and Ion Exchange Chromatography in Waste Water Treatment

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