Retention Behaviour of Synthetic Dyes on Plain and Tri-n-butylamine Impregnated Silica Gel-G and Starch Layers in Aqueous Alcohol Media
S.D. Sharma and Charu Sharma
Retention behaviour of twenty four synthetic dyes on plain and tri-n-butylamine (TBA) impregnated silica gel-G and starch layers in aqueous alcohol media have been studied. Compact and sharp spots led to some useful separations of dyes, having similar chromophore groups on the basis of their differential solubilities and preferential adsorption on these layers. TBA impregnation has significant effect on the resolution of dyes. Diffusion ratios for some dyes were determined. Plain starch layers were found to have the least compactness and, therefore, not much useful for the separation of dyes. The effect of water content in the mobile phase on the development time has been discussed. The easy and inexpensive method can be applied for the trace analysis of synthetic dyes in some natural and synthetic samples.
Retention Behaviour, Synthetic Dyes, TBA Impregnation, Silica Gel and Starch Layers
Published online 11/1/2016, 14 pages
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