The Effect of Some Micro-Elements on Free Amino Acids, Indols and total Phenols Production from Embryogenic Callus of Tow Date Palm Cultivars (Sakkoty and Bartamuda)
Sherif F. El sharabasy, Abdel-Aal W. B., Hussein A. Bosila, Abdel-Monem A. Bana, Bayome M. Mansoudownload PDF
Abstract. Effect of microelements on some chemicals analysis of secondry metabolits such as free amino acids, total indols content and total phenols content of date palm cultivars (Sakkoty and Bartamuda) were study in this work. Different concentrations of manganese sulfate (MnSO4 2 H2O) (22.3, 44.6 and 66.9 mg/l), zinc sulfate (ZnSO47H2O) (8.6, 17.2 and 25.8 mg/l) and copper sulfate (CuSO45H2O) (0.025, 0.050, 0.075 mg/l) were added into nutrient medium of embryogenic callus stage. The results illustrated that, addtion of manganese sulfate at ( 22.3 mg/l) to culture medium of embryonic callus of Bartamoda cv. gave the highest significant values of total free amino acids (1.75 mg/g fresh weight) and (0.33 mg/g fresh weight) of total indols. Where the addition of manganese sulfate at (66.9 mg/l) to nuutrient medium of growing embryogenic callus of Bartamuda cv. gave the highest significant value of total phenols (1.17 mg/g fresh weight). The addititon of zinc sulfate at (17.2 mg/l) to culture medium of embryogenic callus of Sakkoty cv., recorded the highest significant values of total amino acids (1.64 mg/g fresh weight) and Indoles( 0.40 mg/g fresh weight). While the highest significant values of total phenol content was (1.24 mg/g fresh weight) when embryonic callus of Sakkoty cv. grown on medium contained zinc sulfate at (25.8 mg/l). Data showed also the highest significant values of total free amino acids and total indols content (1.36 and 0.40 mg/g fresh weight respectively) were achived when embryogenic callus of Bartamuda cv. was grown on medium containing of copper sulfate at (0.025 mg/l),wherase the highest significant value of total phenols content (1.83 mg/g fresh weight) were recorded when embryonic callus of Sakkoty cv. was grown on nutrient medium supplemented with copper sulfate at (0.075 mg/l).
microelements, manganese sulfate (MnSO4 2 H2O), zinc sulfate (ZnSO47H2O), copper sulfate (CuSO45H2O), amino acids, phenols, indols, embryogenic callus, date palm
Published online 4/20/2019, 9 pages
Copyright © 2019 by the author(s)
Published under license by Materials Research Forum LLC., Millersville PA, USA
Citation: Sherif F. El sharabasy, Abdel-Aal W. B., Hussein A. Bosila, Abdel-Monem A. Bana, Bayome M. Mansou, The Effect of Some Micro-Elements on Free Amino Acids, Indols and total Phenols Production from Embryogenic Callus of Tow Date Palm Cultivars (Sakkoty and Bartamuda), Materials Research Proceedings, Vol. 11, pp 235-243, 2019
The article was published as article 19 of the book By-Products of Palm Trees and Their Applications
Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
 S. Gantait, M.M. El-Dawayati, J. Panigrahi, C. Labrooy, S.K. Verma, The retrospect and prospect of the applications of biotechnology in ( Phoenix dactylifera L.), App. Microbial. Biotech. 102 (2018) 8229-8259. https://doi.org/10.1007/s00253-018-9232-x
 E. Ahmed, M. Arshad, M.Z. Khan, Secondary metabolites and their multidimensional prospective in plant life, J. Pharm. Phyto. 2 (2017) 205-14.
 R. Al-Alawi, J. Al-Mashiqri, J. Al-Nadabi, B. Al-Shihi, Y. Baq, Date palm tree (Phoenix dactylifera L.) natural products and therapeutic options, Front Plant Sci. 8 (2017) 1-12. https://doi.org/10.3389/fpls.2017.00845
 M.I. Dias, M.J. Sousa, R.C. Alves, I.C. Ferreira, Exploring plant tissue culture to improve the production of phenolic compounds: A review, Industrial Crops and Products, 1-82 (2016) 9-22. https://doi.org/10.1016/j.indcrop.2015.12.016
 I. Smetanska, Production of secondary metabolites using plant cell cultures, In Food biotech Springer, Berlin, Heidelberg, (2008) 187-228.
 W. Yue, Q.L. Ming, B. Lin, K. Rahman, C.J. Zheng, T. Han, L.P. Qin, Medicinal plant cell suspension cultures: pharmaceutical applications and high-yielding strategies for the desired secondary metabolites, Critical reviews in biotechnology 36-2 (2016) 215-32. https://doi.org/10.3109/07388551.2014.923986
 N.A. Fadzliana, S. Rogayah, N.A. Shaharuddin, O.A. Janna, Addition of L-Tyrosine to Improve Betalain Production in Red Pitaya Callus, Pertanika J. Tropical Agr. Sci. 40-4 (2017) 521-532.
 I. Smetanska, Production of secondary metabolites using plant cell cultures, In Food biotech Springer, Berlin, Heidelberg (2008) 187-228.
 C. Poschenrieder, J. Allué, R. Tolrà, M. Llugany, J. Barceló, Trace Elements and Plants Secondary Metabolism: Quality and Efficacy of Herbal Products. Trace Elements as Contaminants and Nutrients/еd. by MNV Prasad Published by John Wiley & Sons, Inc., Hoboken, New Jersey 25 (2008) 99-120. https://doi.org/10.1002/9780470370124.ch5
 Z.E., Zayed, Enhanced Indirect Somatic Embryogenesis from Shoot-Tip Explants of Date Palm by Gradual Reductions of 2, 4-D Concentration, In Date Palm Biotechnology Protocols, Humana Press, New York, 1 (2017) 77-88. https://doi.org/10.1007/978-1-4939-7156-5_7
 M.M. El-Dawayati, H.S. Ghazzawy, M. Munir, Somatic embryogenesis enhancement of date palm cultivar Sewi using different types of polyamines and glutamine amino acid concentration under in-vitro solid and liquid media conditions, Int J Biosci 12 (2018) 149–159. https://doi.org/10.12692/ijb/12.1.149-159
 T. Murashige, F. Skoog, A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant 15 (1962) 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
 Rosein, H., A modified ninhydrin coloremetric analysis for amino acids, Archives of Biochemistry and Biophysics, 67 (1957) 10-15. https://doi.org/10.1016/0003-9861(57)90241-2
 P. Larsen, A. Harbo, S. Klungsour, T. Asheim, On the biogenesis of some indol compounds in Acetobacter xylinum. Physiologia Plantarum, 15 (1962) 552-655. https://doi.org/10.1111/j.1399-3054.1962.tb08058.x
 H.D. Danial, C.M. George, Peach seed dormancy in relation to endogenous inhibitors and applied growth substances, Journal of the American Society for Horticultural Science 17 (1972) 651- 654.
 G.W. Snedecor, W.G. Cochran, Statisical Methods, Oxford and J.B.H. Publishing Co., 6th edition, 1980, pp. 507.
 H. Gaosheng, J. Jingming, Production of Useful Secondary Metabolites Through Regulation of Biosynthetic Pathway in Cell and Tissue Suspension Culture of Medicinal Plants, Recent Advances in Plant in vitro Culture, Annarita Leva and Laura M. R. Rinaldi, IntechOpen, (2012). https://doi.org/10.5772/53038