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​24. International Workshop on Single Molecule Spectroscopy and Super-resolution Microscopy in the Life Sciences

Picture of the winner of this year’s Student Award – André Dathe for his presentation on “Single-molecule FRET analysis of enzyme conformations and diffusion of nanostructures in a confocal ABELtrap” and Johan Tornmalm for his presentation on “Transient State Monitoring of Cellular Autofluorescence”.

EasyTau 2 – measure and analyze spectroscopy data in one package
The newly released software package provides now a fully integrated, comprehensive suite of fitting and analytical tools for time-resolved fluorescence spectroscopy applications. With this upgrade, EasyTau 2 becomes a one-stop solution for both acquiring and analyzing data with PicoQuant’s fully automated, modular, and high-end FluoTime 300 spectrometer.
In addition to proving full control over all hardware components of the FluoTime 300, the new fitting module includes all functions that were previously offered by the stand-alone FluoFit Pro program. Both measurement and analysis of the results can be performed in a single, powerful yet user-friendly graphical environment with efficient data management.
Steady-state and time-resolved data can be subjected to basic arithmetic functions (such as addition, normalization, integration) and fitted to determine fluorescence lifetimes or anisotropy. The module offers global decay analysis, tail and iterative reconvolution fitting with non-linear error minimization as well as using various exponential decay (up to fifth order) or rate constant distribution models. The software also allows for rigorous error analysis (bootstrap) as well as generating presentation-ready numerical and graphical output.
MultiHarp 150 – new generation of timing electronics
As the world market leader in TCSPC timing electronics, PicoQuant is proud to announce the MultiHarp 150, its newest generation of bench-top event timers with USB 3.0 interface. Its outstanding data throughput allows to fully exploit the count rate limits of time-resolved single photon experiments. With 4 or 8 detector channels and a common sync input, the MultiHarp 150 is ideally suited for photon coincidence measurements in quantum optics. It is world’s first commercial event timer on the market, which is prepared to be used in a White Rabbit timing network. This feature allows two modules to be synchronized over ranges as high as tens of kilometers, making it the ideal time tagger for long distance quantum entanglement experiments.
16. European Short Course on Time-resolved Fluorescence Spectroscopy
In collaboration with Joseph R. Lakowicz from the Center of Fluorescence Spectroscopy (CFS) in Baltimore (USA), PicoQuant organizes this annual course on the “Principles and Applications of Time-resolved Fluorescence. The aim of the 4 day event is to provide an in-depth introduction into fluorescence spectroscopy and its applications to scientific fields like life and materials science. This year, the course will be held from November 5 to 8, 2018 in Berlin, the capital city of Germany.
The target audience are researchers from academia and industry interested in using fluorescence spectroscopy methods. The course encompasses a series of lectures with accompanying practical sessions focusing on various instrumentation and software. The list of instructors include Joseph R. Lakowicz from CFS, Zygmunt “Karol” Gryczynski from the Texas Christian University, Forth Worth, USA, Rainer Erdmann, Managing Director of PicoQuant, and further senior scientists of PicoQuant, with extensive experience in time-resolved fluorescence spectroscopy. The practical sessions will focus on steady-state and time-resolved measurements as well as data analysis, with instruments provided by Agilent and PicoQuant.
New microscope installed at the University of Leeds
In a joint effort by PicoQuant and JPK Instruments, a highly versatile microscope which combines Atomic Force Microscopy (AFM) with Fluorescence Lifetime Imaging (FLIM) has been installed at the University of Leeds.
This integrated solution enables researchers to obtain structural information from biological samples with resolutions down to the molecular level while recording the fluorescence behavior of a wide variety of probes from the same sample area. Thanks to a seamless integration, the system can provide combined AFM and optical/FLIM information in a very simple and straightforward way.
The installed FLIMbee galvo scanner from PicoQuant provides researchers with high flexibility in scanning speeds, making it possible to not only accurately measure short-lived fluorescence decays but also longer lived species.
We are looking forward to see what fascinating results will be achieved with this system!
Harper Expands Product Offering with New HC Series™ Configurable Rotary Furnaces
Buffalo, NY – Harper International, a world leader in thermal processing solutions for advanced materials,
announces the launch of the HC Series™ Configurable Rotary Furnace product line. Complementing their renowned
custom designed thermal processing systems; these pre-engineered furnaces are advantageous for clients requiring
more economical equipment solutions with quicker lead times.
The HC Series™ are a standard set of Rotary Furnaces that are configured to order and available in a number of
variations, dependent on the client’s specifications for temperature, tube length, heated length, diameter, and
number of zones. Clients have the opportunity to use a product selector on the Harper website in which their desired
specifications are selected and the proper Rotary Furnace design configuration is formulated.
Tornado Spectral Systems Announces Feature-Packed Software Update with SpectralSoft 2.8
Tornado Spectral Systems Inc., a leading manufacturer of Raman spectrometers, released today an enhanced update of its flagship software SpectralSoft. Version 2.8 equips users with essential functionality including security of access and data, multi-channel operation, remote access and control, as well as advancements to boost measurement precision.
SpectralSoft’s new features include the following:
Security Module
– Organizations can be confident in complying with US FDA 21 CFR Part 11 with login access and management of data security.
Productivity Module
– Users can configure and control multi-channel fibre-switching hardware for monitoring up to eight channels.
Industrial Control Module
– The Industrial Control module features a Modbus TCP interface for DCS connectivity.
SpectralSoft 2.8 raises the bar for Raman data acquisition and data quality. In addition to the add-on modules, 2.8 improves productivity via auto-calibration and increases data signal-to-noise ratio.
11th European Short Course on “Time-resolved Microscopy and Correlation Spectroscopy”
10th European Short Course on “Time-resolved Microscopy and Correlation Spectroscopy”
The course held by PicoQuant offered an in-depth introduction into time-resolved microscopy techniques through lectures and practical sessions.
Berlin (Germany), 19 March 2018 – The 10th edition of this course, held from March 6-8, 2018 in Berlin was highly appreciated and found to be inspiring by the 40 participants from universities and industry. Six lectures and five hands-on sessions with instruments from major microscopy companies including Nikon, Olympus, PicoQuant, and Zeiss were given. These sessions covered different methods and procedures used in time-resolved fluorescence microscopy such as FLIM / rapidFLIM, FLIM-FRET, F(L)CS, PLIM for material science, single molecule spectroscopy, and STED super-resolution microscopy.
The course was intended for individuals looking for an in-depth introduction to the principles of time-resolved fluorescence microscopy and its applications to Life Sciences. Attendees were typically professionals who employ or intend to use fluorescence microscopy in their research. Most attendees had some preliminary knowledge of fluorescence, typically in a specialized area. However, individuals from totally different research areas and industry got the opportunity to enter this exciting field in a very effective way and greatly benefited from the experimental sessions.
Next course: 11th European Short Course on “Time-resolved Microscopy and Correlation Spectroscopy”
Date February / March 2019, Location WISTA Campus, Berlin, Germany, Contact PicoQuant GmbH, Kerstin Wicht, http://www.picoquant.com/microscopy-course, trfcourse@picoquant.com
PicoQuant and Ionovation now offer an integrated platform that combines microscopy and force spectroscopy
PicoQuant‘s MicroTime 200 platform is the most flexible and extensible high-end research tool for time-resolved fluorescence microscopy requiring single molecule sensitivity. The system is an excellent choice for many methods, including Pulsed Interleaved Excitation (PIE), Förster Resonance Transfer (FRET), Stimulated Emission Depletion (STED) super-resolution microscopy, Fluorescence (Lifetime) Correlation Spectroscopy (F(L)CS), and anisotropy measurements.
Ionovation’s PicoTweezers system is a compact, robust trapping and force measurement device based on the “optical tweezers” concept which is equipped with real-time video detection. In contrast to common optical tweezers systems, PicoTweezers has no spatial restrictions and is compatible to all sample carriers. The system is well suited for molecular interaction studies, force spectroscopy, cell trapping, rheology and many other applications.
ZEISS shakes hands with PicoQuant – new software integration
ZEISS has recently developed and released a Handshake plug-in for their ZEISS ZEN imaging software. This plug-in enables acquiring time-resolved data with PicoQuant’s LSM Upgrade Kit within the familiar ZEISS ZEN environment.
This integration provides you with a further way to optimize your Fluorescence Lifetime Imaging (FLIM) or Fluorescence Correlation Spectroscopy (FCS) measurement workflow. The subsequent data analysis is performed employing the powerful and versatile tools provided by PicoQuant’s SymPhoTime 64 software package.
The ZEISS ZEN software integration allows setting up and controlling basic confocal, FLIM, FCS and FLCS measurements as well as more complex acquisition schemes such as multi-point FCS, time-series FLIM, or even 3D-FLIM images. All of these advanced acquisition modes can also be combined into a single experiment.
SMILE VIEW™ Map software
Specifically designed for Scanning Electron Microscope users
SMILE VIEW™ Map gives users access to a powerful set of features for visualizing, analyzing and reporting their data including:
• Super-fast 3D reconstruction of surface topography from one or more SEM images
• 3D visualization at any zoom level or angle plus surface metrology
• Image enhancement & colorization: from black and white to color in just a few clicks
• Characterization of surface roughness and texture in accordance with all the latest standards (ISO, JIS, ASME etc.)
• Easy report generation: turn data into accurate, visual analysis reports and export in standard file formats
• Intuitive, interactive workflow for increased throughput
SXR monochromator works 3 different ways
Direct detection CCD detectors and open microchannel plate intensifiers fit. Extreme ultraviolet hollow cathode and solid-anode electron impact light sources do too. They are useful for calibration and spectral test applications and set this instrument apart from others.
Do you have applications for analysis and diagnostics with high energy spectra? The vacuum ultraviolet (VUV) Model 248/310 grazing incidence monochromator fits the bill. Easily make spectral measurements around 13 nanometers and throughout the extreme vacuum ultraviolet and soft x-ray wavelength region. It is ideal for work from 10eV to more than 1000eV.
The Model 248/310 works in diverse laboratory applications. It features one meter focal length optics in a compact Rowland circle instrument. Grazing incidence angles deliver high reflective efficiency. Many diffraction gratings are available to optimize for your application.
UV-Vis and Fluorescence Spectrophotometers Now Offered
Through Eastern Applied Research Inc.
Buffalo, NY – Eastern Applied Research Inc is pleased to be a distributor of UV/Vis and Fluorescence Spectrophotometers from Hitachi High-Tech Science Corporation. The complete line of Hitachi Spectrophotometers, with capabilities fit for teaching environments to research grade requirements, will be available throughout the United States, Canada, and Mexico.
McPherson Appoints Titan Electro-Optics Exclusive Agent in P.R. China and Hong-Kong
McPherson, the leading manufacturer and fabricator of research grade spectrometers, has appointed Titan Electro-Optics Co., Ltd. as Exclusive Agent in P.R. China and Hong-Kong. Customers in these areas can expect an improved buying experience and simpler product selection/specification and importation. Bringing years of experience to the table, Titan EO has well organized, wide spread sales and support teams.
Titan EO’s respected Advanced Photonics team provides expert local service and assistance to customers in education and fundamental research, (clean) energy, efficient lighting and more. The Titan team will introduce new clients to McPherson’s spectroscopy equipment as well as service existing accounts. McPherson sales and engineering teams’ support the Titan agents in the field. During the remainder of 2018, several joint activities are planned. They include spectroscopy technique and instrumentation seminar(s) and support trips by McPherson sales and engineering groups to P.R. China.
Bobcat 640 cameras and Cheetah TE3 camera – from the SWIR to the LWIR realm
Xenics, Europe’s leading developer and manufacturer of advanced infrared detectors, cameras and customized imaging solutions from the SWIR to the LWIR realm, is pleased to announce a significant improvement in the quality of its detectors and resulting cameras.
With the goal of better serving its customers, Xenics has recently increased production capabilities, added extra clean room space, reviewed processing techniques and modernized equipment. First evidence of this improved and upgraded manufacturing process is the significant reduction of dark current in our standard InGaAs SWIR detectors.
The detectors with improved dark current performance will first be introduced in the Bobcat 640 cameras and Cheetah TE3 camera. Later on these detectors will find their way into all InGaAs SWIR cameras, including the vSWIR (visible enhanced short-wave infrared) models with extended response into the visible range.
Hemispherical Analyzer
The RESOLVE120 spectrometer is used for Electron Spectrocopy for Chemical Analysis (ESCA) ; X-ray photoelectron spectroscopy (XPS), Auger electron spectroscopy (AES), ultraviolet photoelectron spectroscopy (UPS), electron loss spectroscopy (ELS) and synchrotron studies.
These surface sensitive analysis techniques are used to analyse the surface chemistry of a solid material and provide elemental composition, chemical state and electronic state information. It is routinely used to analyze elements, inorganic compounds, metal alloys, semiconductors, polymers, catalysts, glasses, ceramics, paints, corrosion, adhesion and thin film coatings.
New PHI Quantes
The new PHI Quantes is a laboratory-based instrument used for performing both traditional XPS measurements using an Al Kα X-ray source, plus extended depth of analysis experiments using a Cr Kα (hard) X-ray source. The latter is referred to as HAXPES – Hard X-ray Photoelectron Spectroscopy. The Cr X-ray source provides for depths of analysis roughly 3 times those obtained using an Al X-ray source. The Quantes is based on the tried and true Quantera XPS platform.
Efficient stability testing of emulsions and dispersions using the Multiscan MS20, DataPhysics Instruments
Emulsions and dispersions like milk, emulsifiers, skin creams, paints and coatings are heterogeneous, colloidal systems which have to be analyzed objectively on their stability and aging behavior in their original condition. Using the DataPhysics MultiScan MS20 such a characterization can be done fully automatically and software driven.
Bobcat-320-Gated – Cost sensitive SWIR InGaAs camera series
The Bobcat-320 is a small, high performance shortwave infrared camera. Its thermoelectric cooler ensures excellent low noise and low dark current characteristics.
The Bobcat-320 targets cost-sensitive volume markets such as laser beam analysis applications, sorting & recycling industry and investigation of defects inside silicon semiconductor material. This shortwave infrared camera comes with a CameraLink or GigE Vision interface and has a low weight, low power consumption and is small in size.
3-gratings for an optical spectrum from 8 to 2000 eV
Shallow 1.5 degree grazing incidence angle improves efficiency for high energy photons, wavelengths below 5 nanometers. Instrument now optimal for energies low as 8 eV (150 nanometers wavelength) and high as 2000 eV (0.6 nanometers).
High energy optical spectroscopy has a new “power tool.” For water window imaging, HHG laser spectroscopy, x-ray plasma diagnostics, extreme ultraviolet lithography, optical characterization, metrology and calibration the McPherson Model 251MX delivers uncompromising performance. The Model 251MX is easy to use and delivers results over the wide 0.5 to 150 nanometer range simply by indexing diffraction gratings. See detailed specifications on the data sheet.
The McPherson Model 251MX is optimized for high energy photons including soft x-ray and extreme UV (EUVL). The Model 251MX does not scan with point detectors, it works exclusively with microchannel plate intensifiers (MCP) and/or direct detection CCD array detectors. Regardless of type, the detector is located on a flat focal plane. The aberration correction provides flat field and straight spectral lines ideal for planar detectors and good spectral resolution. “We see many different types of applications” states Erik Schoeffel, Sales Coordinator at McPherson. “Whether you have photoemission microscopy with light, low energy elements, a ultrafast high harmonic laser, a pulse probe experiment or need to diagnose a free electron laser or hot plasma, the aberration corrected 251MX quickly collect broad swaths of extreme ultraviolet.”
Taiko PDL M1 – Your Rhythm. Our Light.
Take full control over your pulsed picosecond light sources with PicoQuant recently released Taiko PDL M1 smart laser driver. This laser driver is tailor-made for the most demanding applications in life and material science, semiconductor diagnostic as well as laser-ranging.
The Taiko PDL M1 is able to monitor and control most laser head parameters such as optical output power or laser head temperature. It offers unmatched operational flexibility, including a wide range of repetition rates, burst patterns, continuous wave operation, and external triggering. Featuring both an intuitive local one button control scheme and a versatile remote user interface, operating a laser has never been more easy and enjoyable. The two interfaces are fully synchronized and can be used simultaneously.
Triform to Manufacture Fluid Cell Sheet Hydroforming Press for Aerospace Supplier H&R Parts Company
St. Louis, MO – Beckwood Press Company, a leading manufacturer of custom hydraulic presses and automation systems and the Triform line of precision forming equipment, has been awarded a contract by H&R Parts Company to manufacture a Triform model 24-5BD fluid cell sheet hydroforming press. With a 24”-diameter forming area and 5,000 PSI, the new Triform will support H&R’s aluminum aerospace part production.
During the fluid cell process, a pressurized rubber diaphragm filled with hydraulic oil shapes sheet material against a single tool. This evenly-distributed pressure results in net shape part production, virtually eliminating the need for hand-finishing. Due to the universal nature of the Triform diaphragm, which conforms to any shape within the forming chamber, the need for expensive mated male/female dies is eliminated. Additionally, multiple parts can be formed in a single cycle, as long as the tools and blanks fit within the forming area.
Beckwood Engineers Two 13-ton Servo-Driven Electro-Mechanical Presses
Beckwood Press Company, a leading manufacturer of custom hydraulic presses and automation systems and the Triform line of precision forming equipment, has been awarded a contract to manufacture two 13-ton servo-driven electro-mechanical presses for use by a leading footwear manufacturer in a trimming application.
Scheduled for delivery in February 2018, the twin 40” x 50” x 158” presses feature a monolithic frame, quick die clamps, 13” of stroke, and a knockout cylinder mounted inside the ram weldment to strip the part after it is trimmed. Using a Parker XFC electro-mechanical actuator to drive the main ram and a load cell to monitor force feedback, cycle times on these machines will average 2.5 seconds or less.
FocalSpec and Digital Surf team up to launch new FocalSpec Map software
Oulu, Finland & Besançon, France: Finnish high-tech company FocalSpec, specialized in devices for fast surface measurement and on-line testing of challenging materials and shapes, and Digital Surf, global provider of Mountains® surface analysis software solutions, have teamed up to release FocalSpec Map, a cutting-edge software package bringing a whole new set of tools to users of the Finnish company’s innovative Line Confocal Imaging (LCI) technology.
Customers in a wide range of industries (consumer and mobile electronics, plastics and medical devices to name but a few) now have the ability to visualize, analyze and report on measured surface data in greater depth than ever before.
Enhanced Specifications for the PT410
Cryomech is constantly working to improve the products they offer to you. They strive to innovate to meet customers’ needs and provide unique solutions for their specific applications. With that said, we are pleased to announce enhanced specifications for the PT410 Pulse Tube Cryorefrigerators.
Short video on the HyperFlux™ PRO Plus spectrometer
To demonstrate the sensitivity of the PRO Plus for making real-time process measurements or for reaction monitoring, we’ve performed a simple experiment where we add small quantities of propylene glycol to water and then monitor the mixture with a PRO Plus analyzer and MarqMetrix immersion probe.
The new McPherson Model 207V
The new model will be an important part of many deep-UV and VUV imaging and analytical spectroscopy systems. It works over a broad spectral range, from the deep UV, through the Visible into the long-wave Infrared. Its clean construction is ideal for contaminant free purge and vacuum applications.
670mm focal length monochromator / spectrometer, McPherson Model 207
PI-KEM Limited latest product news
Graphene
We have recently expanded our vast materials range to cover the new ‘wonder material’ Graphene. Following new partnerships with Graphenea and Advanced Graphene Products we now offer:
• Graphene Oxide
• Reduced Graphene Oxide
• CVD Films
• HSMGTM High Strength Metallurgical GrapheneTM
Covering applications and research within – Batteries, energy, electronics, supercapacitors, surface chemistry, thermal management, flexible electronics, flexible OLED, composites, biomedical materials, biosensors, carbon nanotubes, ceramics, car industry, cleantech, electric vehicles, European project, aviation, space industry, nanopores, nanoribbons, photonics, photodetector, plasmons, quantum dots, transistors, transparent conductor, mobile devices, nanotechnology, solar cells.
New improved stability windowless hollow cathode UV lamp
High-energy UV lamp
McPherson is pleased to announce new and improved stability with flow controlled McPherson windowless, hollow cathode UV lamp Model 629. This broad-spectrum source emits ionized gas emission lines with little or no absorption by neutral gas. Computer controlled gas flow and constant current power supply improve stability for better spectroscopy.
windowless gas ionizaion uv lamp for calibrating spectrometers
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